A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax

Bruce Russell; Rossarin Suwanarusk; Céline Borlon; Fabio T M Costa; Cindy S Chu; Marcus J Rijken; Kanlaya Sriprawat; Lucile Warter; Esther G L Koh; Benoit Malleret; Yves Colin; Olivier Bertrand; John H Adams; Umberto D'Alessandro; Georges Snounou; Francois Nosten; Laurent Rénia

doi:10.1182/blood-2011-04-348748

A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax

Bruce Russell, Rossarin Suwanarusk, Céline Borlon, Fabio T M Costa, Cindy S Chu, Marcus J Rijken, Kanlaya Sriprawat, Lucile Warter, Esther G L Koh, Benoit Malleret, Yves Colin, Olivier Bertrand, John H Adams, Umberto D'Alessandro, Georges Snounou, Francois Nosten, Laurent Rénia

Birth Center Obstetrics - Medical

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand.

Original language	English
Pages (from-to)	e74-81
Journal	Blood
Volume	118
Issue number	13
DOIs	https://doi.org/10.1182/blood-2011-04-348748
Publication status	Published - 29 Sept 2011

Keywords

Cells, Cultured
Hematologic Tests
High-Throughput Screening Assays
Host-Pathogen Interactions
Humans
Malaria, Vivax
Plasmodium vivax
Reproducibility of Results
Reticulocytes
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't
Validation Studies

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10.1182/blood-2011-04-348748

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Russell, B., Suwanarusk, R., Borlon, C., Costa, F. T. M., Chu, C. S., Rijken, M. J., Sriprawat, K., Warter, L., Koh, E. G. L., Malleret, B., Colin, Y., Bertrand, O., Adams, J. H., D'Alessandro, U., Snounou, G., Nosten, F., & Rénia, L. (2011). A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax. Blood, 118(13), e74-81. https://doi.org/10.1182/blood-2011-04-348748

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title = "A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax",

abstract = "Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand.",

keywords = "Cells, Cultured, Hematologic Tests, High-Throughput Screening Assays, Host-Pathogen Interactions, Humans, Malaria, Vivax, Plasmodium vivax, Reproducibility of Results, Reticulocytes, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't, Validation Studies",

author = "Bruce Russell and Rossarin Suwanarusk and C{\'e}line Borlon and Costa, {Fabio T M} and Chu, {Cindy S} and Rijken, {Marcus J} and Kanlaya Sriprawat and Lucile Warter and Koh, {Esther G L} and Benoit Malleret and Yves Colin and Olivier Bertrand and Adams, {John H} and Umberto D'Alessandro and Georges Snounou and Francois Nosten and Laurent R{\'e}nia",

year = "2011",

month = sep,

day = "29",

doi = "10.1182/blood-2011-04-348748",

language = "English",

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pages = "e74--81",

journal = "Blood",

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Russell, B, Suwanarusk, R, Borlon, C, Costa, FTM, Chu, CS, Rijken, MJ, Sriprawat, K, Warter, L, Koh, EGL, Malleret, B, Colin, Y, Bertrand, O, Adams, JH, D'Alessandro, U, Snounou, G, Nosten, F & Rénia, L 2011, 'A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax', Blood, vol. 118, no. 13, pp. e74-81. https://doi.org/10.1182/blood-2011-04-348748

TY - JOUR

T1 - A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax

AU - Russell, Bruce

AU - Suwanarusk, Rossarin

AU - Borlon, Céline

AU - Costa, Fabio T M

AU - Chu, Cindy S

AU - Rijken, Marcus J

AU - Sriprawat, Kanlaya

AU - Warter, Lucile

AU - Koh, Esther G L

AU - Malleret, Benoit

AU - Colin, Yves

AU - Bertrand, Olivier

AU - Adams, John H

AU - D'Alessandro, Umberto

AU - Snounou, Georges

AU - Nosten, Francois

AU - Rénia, Laurent

PY - 2011/9/29

Y1 - 2011/9/29

N2 - Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand.

AB - Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand.

KW - Cells, Cultured

KW - Hematologic Tests

KW - High-Throughput Screening Assays

KW - Host-Pathogen Interactions

KW - Humans

KW - Malaria, Vivax

KW - Plasmodium vivax

KW - Reproducibility of Results

KW - Reticulocytes

KW - Evaluation Studies

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Validation Studies

U2 - 10.1182/blood-2011-04-348748

DO - 10.1182/blood-2011-04-348748

M3 - Article

C2 - 21768300

SN - 0006-4971

VL - 118

SP - e74-81

JO - Blood

JF - Blood

IS - 13

ER -

A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax

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Keywords

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