A recombinant, fully human monoclonal antibody with antitumor activity constructed from phage-displayed antibody fragments

G. Huls; I.A.F.M. Heijnen; M.E. Cuomo; J.C. Koningsberger; L.J. Wiegman; E. Boel; A.R. van der Vuurst de Vries; S.A.J. Loyson; W. Helfrich; G.P. van Berge Henegouwen; M. van Meijer; C.A. de Kruif; T. Logtenberg

doi:10.1038/7023

A recombinant, fully human monoclonal antibody with antitumor activity constructed from phage-displayed antibody fragments

G. Huls, I.A.F.M. Heijnen, M.E. Cuomo, J.C. Koningsberger, L.J. Wiegman, E. Boel, A.R. van der Vuurst de Vries, S.A.J. Loyson, W. Helfrich, G.P. van Berge Henegouwen, M. van Meijer, C.A. de Kruif, T. Logtenberg

Research output: Contribution to journal › Article › Academic › peer-review

81 Citations (Scopus)

Abstract

A single-chain Fv antibody fragment specific for the tumor-associated Ep-CAM molecule was isolated from a semisynthetic phage display library and converted into an intact, fully human IgG1 monoclonal antibody (huMab). The purified huMab had an affinity of 5 nM and effectively mediated tumor cell killing in in vitro and in vivo assays. These experiments show that nonimmunized phage antibody display libraries can be used to obtain high- affinity, functional, and clinically applicable huMabs directed against a tumor-associated antigen.

Original language	English
Pages (from-to)	276-281
Number of pages	6
Journal	Nature Biotechnology
Volume	17
Issue number	3
DOIs	https://doi.org/10.1038/7023
Publication status	Published - 1999

Keywords

Ep-CAM
Immunotherapy
Phage display
Recombinant antibodies
Single-chain Fv

Access to Document

10.1038/7023

Cite this

Huls, G., Heijnen, I. A. F. M., Cuomo, M. E., Koningsberger, J. C., Wiegman, L. J., Boel, E., van der Vuurst de Vries, A. R., Loyson, S. A. J., Helfrich, W., van Berge Henegouwen, G. P., van Meijer, M., de Kruif, C. A., & Logtenberg, T. (1999). A recombinant, fully human monoclonal antibody with antitumor activity constructed from phage-displayed antibody fragments. Nature Biotechnology, 17(3), 276-281. https://doi.org/10.1038/7023

@article{0f607e0077274e98affb7023c482d0a3,

title = "A recombinant, fully human monoclonal antibody with antitumor activity constructed from phage-displayed antibody fragments",

abstract = "A single-chain Fv antibody fragment specific for the tumor-associated Ep-CAM molecule was isolated from a semisynthetic phage display library and converted into an intact, fully human IgG1 monoclonal antibody (huMab). The purified huMab had an affinity of 5 nM and effectively mediated tumor cell killing in in vitro and in vivo assays. These experiments show that nonimmunized phage antibody display libraries can be used to obtain high- affinity, functional, and clinically applicable huMabs directed against a tumor-associated antigen.",

keywords = "Ep-CAM, Immunotherapy, Phage display, Recombinant antibodies, Single-chain Fv",

author = "G. Huls and I.A.F.M. Heijnen and M.E. Cuomo and J.C. Koningsberger and L.J. Wiegman and E. Boel and \{van der Vuurst de Vries\}, A.R. and S.A.J. Loyson and W. Helfrich and \{van Berge Henegouwen\}, G.P. and \{van Meijer\}, M. and \{de Kruif\}, C.A. and T. Logtenberg",

year = "1999",

doi = "10.1038/7023",

language = "English",

volume = "17",

pages = "276--281",

journal = "Nature Biotechnology",

issn = "1087-0156",

publisher = "Nature Research",

number = "3",

}

Huls, G, Heijnen, IAFM, Cuomo, ME, Koningsberger, JC, Wiegman, LJ, Boel, E, van der Vuurst de Vries, AR, Loyson, SAJ, Helfrich, W, van Berge Henegouwen, GP, van Meijer, M, de Kruif, CA & Logtenberg, T 1999, 'A recombinant, fully human monoclonal antibody with antitumor activity constructed from phage-displayed antibody fragments', Nature Biotechnology, vol. 17, no. 3, pp. 276-281. https://doi.org/10.1038/7023

TY - JOUR

T1 - A recombinant, fully human monoclonal antibody with antitumor activity constructed from phage-displayed antibody fragments

AU - Huls, G.

AU - Heijnen, I.A.F.M.

AU - Cuomo, M.E.

AU - Koningsberger, J.C.

AU - Wiegman, L.J.

AU - Boel, E.

AU - van der Vuurst de Vries, A.R.

AU - Loyson, S.A.J.

AU - Helfrich, W.

AU - van Berge Henegouwen, G.P.

AU - van Meijer, M.

AU - de Kruif, C.A.

AU - Logtenberg, T.

PY - 1999

Y1 - 1999

N2 - A single-chain Fv antibody fragment specific for the tumor-associated Ep-CAM molecule was isolated from a semisynthetic phage display library and converted into an intact, fully human IgG1 monoclonal antibody (huMab). The purified huMab had an affinity of 5 nM and effectively mediated tumor cell killing in in vitro and in vivo assays. These experiments show that nonimmunized phage antibody display libraries can be used to obtain high- affinity, functional, and clinically applicable huMabs directed against a tumor-associated antigen.

AB - A single-chain Fv antibody fragment specific for the tumor-associated Ep-CAM molecule was isolated from a semisynthetic phage display library and converted into an intact, fully human IgG1 monoclonal antibody (huMab). The purified huMab had an affinity of 5 nM and effectively mediated tumor cell killing in in vitro and in vivo assays. These experiments show that nonimmunized phage antibody display libraries can be used to obtain high- affinity, functional, and clinically applicable huMabs directed against a tumor-associated antigen.

KW - Ep-CAM

KW - Immunotherapy

KW - Phage display

KW - Recombinant antibodies

KW - Single-chain Fv

UR - https://www.scopus.com/pages/publications/0033058420

U2 - 10.1038/7023

DO - 10.1038/7023

M3 - Article

C2 - 10096296

SN - 1087-0156

VL - 17

SP - 276

EP - 281

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 3

ER -

A recombinant, fully human monoclonal antibody with antitumor activity constructed from phage-displayed antibody fragments

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this