TY - JOUR
T1 - A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles
AU - Jiang, Linglei
AU - Luirink, Joen
AU - Kooijmans, Sander A A
AU - van Kessel, Kok P M
AU - Jong, Wouter
AU - van Essen, Max
AU - Seinen, Cor W
AU - de Maat, Steven
AU - de Jong, Olivier G
AU - Gitz-François, Jerney F F
AU - Hennink, Wim E
AU - Vader, Pieter
AU - Schiffelers, Raymond M
N1 - Funding Information:
L.J. would like to thank PhD Candidate Dennis Doorduijn (Department of medical microbiology, UMC Utrecht, the Netherlands) for useful discussion. L.J. is supported by a personal grant from the CSC.
Funding Information:
L.J. would like to thank PhD Candidate Dennis Doorduijn (Department of medical microbiology, UMC Utrecht, the Netherlands) for useful discussion. L.J. is supported by a personal grant from the CSC.
Publisher Copyright:
© 2020 The Authors
PY - 2021/4
Y1 - 2021/4
N2 - Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a 'post-insertion' approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.
AB - Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a 'post-insertion' approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.
KW - Extracellular vesicles
KW - Outer membrane vesicles
KW - Poly(ethylene glycol)lipids
KW - Post-insertion
UR - http://www.scopus.com/inward/record.url?scp=85093682922&partnerID=8YFLogxK
U2 - 10.1016/j.bbagen.2020.129763
DO - 10.1016/j.bbagen.2020.129763
M3 - Article
C2 - 33065252
SN - 0304-4165
VL - 1865
JO - Biochimica et biophysica acta-General subjects
JF - Biochimica et biophysica acta-General subjects
IS - 4
M1 - 129763
ER -