A Plasma Creatinine- and Urea-Based Equation to Estimate Glomerular Filtration Rate in Rats

Paul J Besseling; Tobias Theodorus Pieters; Isabel T N Nguyen; Petra M de Bree; Nel Willekes; Adele H Dijk; Dominique M Bovée; Ewout J Hoorn; Maarten B Rookmaaker; Karin G Gerritsen; Marianne C Verhaar; Hendrik Gremmels; Jaap A Joles

doi:10.1152/ajprenal.00656.2020

A Plasma Creatinine- and Urea-Based Equation to Estimate Glomerular Filtration Rate in Rats

Paul J Besseling, Tobias Theodorus Pieters, Isabel T N Nguyen, Petra M de Bree, Nel Willekes, Adele H Dijk, Dominique M Bovée, Ewout J Hoorn, Maarten B Rookmaaker, Karin G Gerritsen, Marianne C Verhaar, Hendrik Gremmels, Jaap A Joles

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of glomerular filtration rate (GFR) with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine- and plasma urea-based equation to estimate GFR in a large cohort of male rats [development cohort n = 325, R 2 = 0.816, percentage of predictions that fell within 30% of the true value (P30) = 76%] that had high accuracy in the validation cohort ( n = 116 rats, R 2 = 0.935, P30 = 79%). The equation was less accurate in rats with nonsteady-state creatinine, in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. NEW & NOTEWORTHY This is the first equation, that we know of, which estimates glomerular filtration rate in rats based on a single measurement of body weight, plasma creatinine, and plasma urea.

Original language	English
Pages (from-to)	F518-F524
Journal	American journal of physiology. Renal physiology
Volume	320
Issue number	3
Early online date	1 Feb 2021
DOIs	https://doi.org/10.1152/ajprenal.00656.2020
Publication status	Published - Mar 2021

Keywords

Creatinine
Estimated glomerular filtration rate
Inulin clearance
Rats
Urea

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Besseling, P. J., Pieters, T. T., Nguyen, I. T. N., de Bree, P. M., Willekes, N., Dijk, A. H., Bovée, D. M., Hoorn, E. J., Rookmaaker, M. B., Gerritsen, K. G., Verhaar, M. C., Gremmels, H., & Joles, J. A. (2021). A Plasma Creatinine- and Urea-Based Equation to Estimate Glomerular Filtration Rate in Rats. American journal of physiology. Renal physiology, 320(3), F518-F524. https://doi.org/10.1152/ajprenal.00656.2020

@article{316aeadbb72342f192a9064af01b65ac,

title = "A Plasma Creatinine- and Urea-Based Equation to Estimate Glomerular Filtration Rate in Rats",

abstract = "Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of glomerular filtration rate (GFR) with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine- and plasma urea-based equation to estimate GFR in a large cohort of male rats [development cohort n = 325, R 2 = 0.816, percentage of predictions that fell within 30% of the true value (P30) = 76%] that had high accuracy in the validation cohort ( n = 116 rats, R 2 = 0.935, P30 = 79%). The equation was less accurate in rats with nonsteady-state creatinine, in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. NEW & NOTEWORTHY This is the first equation, that we know of, which estimates glomerular filtration rate in rats based on a single measurement of body weight, plasma creatinine, and plasma urea. ",

keywords = "Creatinine, Estimated glomerular filtration rate, Inulin clearance, Rats, Urea",

author = "Besseling, {Paul J} and Pieters, {Tobias Theodorus} and Nguyen, {Isabel T N} and {de Bree}, {Petra M} and Nel Willekes and Dijk, {Adele H} and Bov{\'e}e, {Dominique M} and Hoorn, {Ewout J} and Rookmaaker, {Maarten B} and Gerritsen, {Karin G} and Verhaar, {Marianne C} and Hendrik Gremmels and Joles, {Jaap A}",

note = "Funding Information: This work was supported by The Netherlands CardioVascular Research Initiative: an initiative with support of the Dutch Heart Foundation [Grant No. CVON2014-11 (RECONNECT)], by ZonMw within the LSH 2Treat programme and the Dutch Kidney Foundation [Grant No. 436001003 (InSiTeVx)], and by the Alexandre Suerman Stipend from UMC Utrecht. Publisher Copyright: {\textcopyright} 2021 the American Physiological Society",

year = "2021",

month = mar,

doi = "10.1152/ajprenal.00656.2020",

language = "English",

volume = "320",

pages = "F518--F524",

journal = "American journal of physiology. Renal physiology",

issn = "1931-857X",

publisher = "American Physiological Society",

number = "3",

}

Besseling, PJ, Pieters, TT, Nguyen, ITN, de Bree, PM, Willekes, N, Dijk, AH, Bovée, DM, Hoorn, EJ, Rookmaaker, MB , Gerritsen, KG , Verhaar, MC, Gremmels, H & Joles, JA 2021, 'A Plasma Creatinine- and Urea-Based Equation to Estimate Glomerular Filtration Rate in Rats', American journal of physiology. Renal physiology, vol. 320, no. 3, pp. F518-F524. https://doi.org/10.1152/ajprenal.00656.2020

TY - JOUR

T1 - A Plasma Creatinine- and Urea-Based Equation to Estimate Glomerular Filtration Rate in Rats

AU - Besseling, Paul J

AU - Pieters, Tobias Theodorus

AU - Nguyen, Isabel T N

AU - de Bree, Petra M

AU - Willekes, Nel

AU - Dijk, Adele H

AU - Bovée, Dominique M

AU - Hoorn, Ewout J

AU - Rookmaaker, Maarten B

AU - Gerritsen, Karin G

AU - Verhaar, Marianne C

AU - Gremmels, Hendrik

AU - Joles, Jaap A

N1 - Funding Information: This work was supported by The Netherlands CardioVascular Research Initiative: an initiative with support of the Dutch Heart Foundation [Grant No. CVON2014-11 (RECONNECT)], by ZonMw within the LSH 2Treat programme and the Dutch Kidney Foundation [Grant No. 436001003 (InSiTeVx)], and by the Alexandre Suerman Stipend from UMC Utrecht. Publisher Copyright: © 2021 the American Physiological Society

PY - 2021/3

Y1 - 2021/3

N2 - Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of glomerular filtration rate (GFR) with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine- and plasma urea-based equation to estimate GFR in a large cohort of male rats [development cohort n = 325, R 2 = 0.816, percentage of predictions that fell within 30% of the true value (P30) = 76%] that had high accuracy in the validation cohort ( n = 116 rats, R 2 = 0.935, P30 = 79%). The equation was less accurate in rats with nonsteady-state creatinine, in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. NEW & NOTEWORTHY This is the first equation, that we know of, which estimates glomerular filtration rate in rats based on a single measurement of body weight, plasma creatinine, and plasma urea.

AB - Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of glomerular filtration rate (GFR) with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine- and plasma urea-based equation to estimate GFR in a large cohort of male rats [development cohort n = 325, R 2 = 0.816, percentage of predictions that fell within 30% of the true value (P30) = 76%] that had high accuracy in the validation cohort ( n = 116 rats, R 2 = 0.935, P30 = 79%). The equation was less accurate in rats with nonsteady-state creatinine, in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. NEW & NOTEWORTHY This is the first equation, that we know of, which estimates glomerular filtration rate in rats based on a single measurement of body weight, plasma creatinine, and plasma urea.

KW - Creatinine

KW - Estimated glomerular filtration rate

KW - Inulin clearance

KW - Rats

KW - Urea

UR - http://www.scopus.com/inward/record.url?scp=85102964212&partnerID=8YFLogxK

U2 - 10.1152/ajprenal.00656.2020

DO - 10.1152/ajprenal.00656.2020

M3 - Article

C2 - 33522412

SN - 1931-857X

VL - 320

SP - F518-F524

JO - American journal of physiology. Renal physiology

JF - American journal of physiology. Renal physiology

IS - 3

ER -

A Plasma Creatinine- and Urea-Based Equation to Estimate Glomerular Filtration Rate in Rats

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