TY - JOUR
T1 - A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells
AU - Jain Goyal, Manu
AU - Zhao, Xiyan
AU - Bozhinova, Mariya
AU - Andrade-López, Karla
AU - de Heus, Cecilia
AU - Schulze-Dramac, Sandra
AU - Müller-McNicoll, Michaela
AU - Klumperman, Judith
AU - Béthune, Julien
N1 - Funding Information:
We thank Felix Wieland (Heidelberg) for the kind gifts of plasmids and antibodies (see Supplemental Data). We thank Ina Poser (Dresden) for BAC tagging and Menna Ahmed for her help in handling the IncuCyte device. We thank the CellNetworks EM, FACS, and Nikon imaging center facilities for their expert support. We thank F Wieland and Mandy Jeske for their critical comments on the manuscript. This work was supported by the excellence initiative of the German federal and state governments (DFG-EXC81).
Publisher Copyright:
© 2020 Goyal et al.
PY - 2020/9
Y1 - 2020/9
N2 - Coat protein complex I (COPI)-coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether paralogous COPI proteins have specific functions is currently unknown. Here, we show that the paralogous coatomer subunits γ1-COP and γ2-COP are differentially expressed during the neuronal differentiation of mouse pluripotent cells. Moreover, through a combination of genome editing experiments, we demonstrate that whereas γ-COP paralogs are largely functionally redundant, γ1-COP specifically promotes neurite outgrowth. Our work stresses a role of the COPI pathway in neuronal polarization and provides evidence for distinct functions for coatomer paralogous subunits in this process.
AB - Coat protein complex I (COPI)-coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether paralogous COPI proteins have specific functions is currently unknown. Here, we show that the paralogous coatomer subunits γ1-COP and γ2-COP are differentially expressed during the neuronal differentiation of mouse pluripotent cells. Moreover, through a combination of genome editing experiments, we demonstrate that whereas γ-COP paralogs are largely functionally redundant, γ1-COP specifically promotes neurite outgrowth. Our work stresses a role of the COPI pathway in neuronal polarization and provides evidence for distinct functions for coatomer paralogous subunits in this process.
UR - http://www.scopus.com/inward/record.url?scp=85088050880&partnerID=8YFLogxK
U2 - 10.26508/lsa.202000714
DO - 10.26508/lsa.202000714
M3 - Article
C2 - 32665377
VL - 3
JO - Life Science Alliance
JF - Life Science Alliance
IS - 9
M1 - e202000714
ER -