A Ninhydrin-Type Urea Sorbent for the Development of a Wearable Artificial Kidney

Jacobus A W Jong, Yong Guo, Diënty Hazenbrink, Stefania Douka, Dennis Verdijk, Johan van der Zwan, Klaartje Houben, Marc Baldus, Karina C Scheiner, Remco Dalebout, Marianne C Verhaar, Robert Smakman, Wim E Hennink, Karin G F Gerritsen, Cornelus F van Nostrum

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Abstract

The aim of this study is to develop polymeric chemisorbents with a high density of ninhydrin groups, able to covalently bind urea under physiological conditions and thus potentially suitable for use in a wearable artificial kidney. Macroporous beads are prepared by suspension polymerization of 5-vinyl-1-indanone (vinylindanone) using a 90:10 (v/v) mixture of toluene and nitrobenzene as a porogen. The indanone groups are subsequently oxidized in a one-step procedure into ninhydrin groups. Their urea absorption kinetics are evaluated under both static and dynamic conditions at 37 °C in simulated dialysate (urea in phosphate buffered saline). Under static conditions and at a 1:1 molar ratio of ninhydrin: urea the sorbent beads remove ≈0.6-0.7 mmol g-1 and under dynamic conditions and at a 2:1 molar excess of ninhydrin ≈0.6 mmol urea g-1 sorbent in 8 h at 37 °C, which is a step toward a wearable artificial kidney.

Original languageEnglish
Article number1900396
Pages (from-to)e1900396
Number of pages11
JournalMacromolecular Bioscience
Volume20
Issue number3
Early online date17 Feb 2020
DOIs
Publication statusPublished - 1 Mar 2020

Keywords

  • chemisorption
  • dialysis
  • ninhydrin
  • sorbent
  • urea

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