TY - JOUR
T1 - A Highly Ordered, Nanostructured Fluorinated CaP-Coated Melt Electrowritten Scaffold for Periodontal Tissue Regeneration
AU - Daghrery, Arwa
AU - Ferreira, Jessica A.
AU - de Souza Araújo, Isaac J.
AU - Clarkson, Brian H.
AU - Eckert, George J.
AU - Bhaduri, Sarit B.
AU - Malda, Jos
AU - Bottino, Marco C.
N1 - Funding Information:
M.C.B. acknowledges the National Institutes of Health (NIH—National Institute of Dental and Craniofacial Research, grants K08DE023552 and R01DE026578), the OsteoScience Foundation (Peter Geistlich Research Award), the International Association for Dental Research (IADR‐GSK Innovation in Oral Care Award), and the American Academy of Implant Dentistry Foundation (AAIDF). The authors are indebted to Sywe‐Ren Chang for his assistance with the coating experiments and for sharing his knowledge. The authors appreciatively acknowledge Cris Strayhorn and Michelle Lynch (University of Michigan School of Dentistry) for their assistance in preparation of histology (Histology Core) and microCT (MicroCT Core), respectively. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Funding Information:
M.C.B. acknowledges the National Institutes of Health (NIH?National Institute of Dental and Craniofacial Research, grants K08DE023552 and R01DE026578), the OsteoScience Foundation (Peter Geistlich Research Award), the International Association for Dental Research (IADR-GSK Innovation in Oral Care Award), and the American Academy of Implant Dentistry Foundation (AAIDF). The authors are indebted to Sywe-Ren Chang for his assistance with the coating experiments and for sharing his knowledge. The authors appreciatively acknowledge Cris Strayhorn and Michelle Lynch (University of Michigan School of Dentistry) for their assistance in preparation of histology (Histology Core) and microCT (MicroCT Core), respectively. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021/11/3
Y1 - 2021/11/3
N2 - Periodontitis is a chronic inflammatory, bacteria-triggered disorder affecting nearly half of American adults. Although some level of tissue regeneration is realized, its low success in complex cases demands superior strategies to amplify regenerative capacity. Herein, highly ordered scaffolds are engineered via Melt ElectroWriting (MEW), and the effects of strand spacing, as well as the presence of a nanostructured fluorinated calcium phosphate (F/CaP) coating on the adhesion/proliferation, and osteogenic differentiation of human-derived periodontal ligament stem cells, are investigated. Upon initial cell-scaffold interaction screening aimed at defining the most suitable design, MEW poly(ε-caprolactone) scaffolds with 500 µm strand spacing are chosen. Following an alkali treatment, scaffolds are immersed in a pre-established solution to allow for coating formation. The presence of a nanostructured F/CaP coating leads to a marked upregulation of osteogenic genes and attenuated bacterial growth. In vivo findings confirm that the F/CaP-coated scaffolds are biocompatible and lead to periodontal regeneration when implanted in a rat mandibular periodontal fenestration defect model. In aggregate, it is considered that this work can contribute to the development of personalized scaffolds capable of enabling tissue-specific differentiation of progenitor cells, and thus guide simultaneous and coordinated regeneration of soft and hard periodontal tissues, while providing antimicrobial protection.
AB - Periodontitis is a chronic inflammatory, bacteria-triggered disorder affecting nearly half of American adults. Although some level of tissue regeneration is realized, its low success in complex cases demands superior strategies to amplify regenerative capacity. Herein, highly ordered scaffolds are engineered via Melt ElectroWriting (MEW), and the effects of strand spacing, as well as the presence of a nanostructured fluorinated calcium phosphate (F/CaP) coating on the adhesion/proliferation, and osteogenic differentiation of human-derived periodontal ligament stem cells, are investigated. Upon initial cell-scaffold interaction screening aimed at defining the most suitable design, MEW poly(ε-caprolactone) scaffolds with 500 µm strand spacing are chosen. Following an alkali treatment, scaffolds are immersed in a pre-established solution to allow for coating formation. The presence of a nanostructured F/CaP coating leads to a marked upregulation of osteogenic genes and attenuated bacterial growth. In vivo findings confirm that the F/CaP-coated scaffolds are biocompatible and lead to periodontal regeneration when implanted in a rat mandibular periodontal fenestration defect model. In aggregate, it is considered that this work can contribute to the development of personalized scaffolds capable of enabling tissue-specific differentiation of progenitor cells, and thus guide simultaneous and coordinated regeneration of soft and hard periodontal tissues, while providing antimicrobial protection.
KW - 3D printing
KW - bones
KW - melt electrowriting
KW - periodontal regeneration
KW - periodontitis
KW - scaffolds
UR - http://www.scopus.com/inward/record.url?scp=85111697444&partnerID=8YFLogxK
U2 - 10.1002/adhm.202101152
DO - 10.1002/adhm.202101152
M3 - Article
C2 - 34342173
AN - SCOPUS:85111697444
SN - 2192-2640
VL - 10
SP - 1
EP - 19
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
IS - 21
M1 - 2101152
ER -