TY - JOUR
T1 - A flow cytometric rosetting assay for the analysis of Fc receptors and C3 receptors on HSV-infected cells
AU - Van Vliet, Karin E.
AU - De Graaf-Miltenburg, Lia A.M.
AU - Verhoef, Jan
AU - Van Strijp, Jos A.G.
PY - 1993/1/4
Y1 - 1993/1/4
N2 - A sensitive and reproducible flow cytometric assay was developed for the analysis of Fcγ and C3b(i) receptors on HSV-infected cells. The method is based on a rosette technique using fluorochrome-labeled erythrocytes sensitized with IgG or C3b(i). A comparison of flow cytometric and microscopic quantitation demonstrated that the binding of EIgG, EC3b(i) to HSV-infected cells were correlated. Flow cytometric analysis provides the opportunity to study simultaneously the distribution of E per HSV-infected cell and the total binding of E to the whole population of HSV-infected cells. Receptor activity and HSV glycoprotein cell surface expression were shown to be correlated in a linear fashion. The assay could be applied to other FcγR- and C3b(i)R-bearing cells.
AB - A sensitive and reproducible flow cytometric assay was developed for the analysis of Fcγ and C3b(i) receptors on HSV-infected cells. The method is based on a rosette technique using fluorochrome-labeled erythrocytes sensitized with IgG or C3b(i). A comparison of flow cytometric and microscopic quantitation demonstrated that the binding of EIgG, EC3b(i) to HSV-infected cells were correlated. Flow cytometric analysis provides the opportunity to study simultaneously the distribution of E per HSV-infected cell and the total binding of E to the whole population of HSV-infected cells. Receptor activity and HSV glycoprotein cell surface expression were shown to be correlated in a linear fashion. The assay could be applied to other FcγR- and C3b(i)R-bearing cells.
KW - C3b receptor
KW - C3bi receptor
KW - Fc receptor
KW - Flow cytometric assay
KW - Herpes simplex virus type 1
UR - http://www.scopus.com/inward/record.url?scp=0027471829&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(93)90070-N
DO - 10.1016/0022-1759(93)90070-N
M3 - Article
C2 - 8423374
AN - SCOPUS:0027471829
SN - 0022-1759
VL - 157
SP - 57
EP - 64
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -