A distal Sp1-element is necessary for maximal activity of the human gastrin gene promoter

J R Bundgaard, T O Hansen, L Friis-Hansen, I J Rourke, W W van Solinge, F C Nielsen, J F Rehfeld

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Studies of transgenic mice have shown that transcriptional control of the gastrin gene exhibits significant species differences. Transfection of the human gastrin promoter in murine cells have depicted proximal Sp1, E-box and CACC elements as the major determinants of transcription. We have examined cis-regulatory elements of the human promoter on a human gastrin expressing cell line and find that a distal -135 to -142 Sp1 element is necessary for maximal activity. Alignment of the mouse and human promoters shows that the proximal human Sp1 and CACC elements are not conserved, whereas the E-box element is retained. The distal Sp1 element is present in mouse but exhibits a C to T transition in the core that is likely to reduce binding affinity of Sp1. We conclude that gastrin gene transcription is regulated by distinct elements in man and rodents.

Original languageEnglish
Pages (from-to)225-8
Number of pages4
JournalFEBS letters
Volume369
Issue number2-3
Publication statusPublished - 7 Aug 1995

Keywords

  • Adenocarcinoma
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA/metabolism
  • Gastrins/genetics
  • Humans
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic/genetics
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Deletion
  • Sp1 Transcription Factor/metabolism
  • Stomach Neoplasms
  • Transcription, Genetic/genetics
  • Tumor Cells, Cultured

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