A comparative analysis by SAGE of gene expression profiles of Barrett's esophagus, normal squamous esophagus, and gastric cardia

JWPM Van Baal; F Milano; AM Rygiel; JJGHM Bergman; WD Rosmolen; SJH Van Deventer; KK Wang; MP Peppelenbosch; KK Krishnadath

doi:10.1053/j.gastro.2005.07.026

A comparative analysis by SAGE of gene expression profiles of Barrett's esophagus, normal squamous esophagus, and gastric cardia

JWPM Van Baal, F Milano, AM Rygiel, JJGHM Bergman, WD Rosmolen, SJH Van Deventer, KK Wang, MP Peppelenbosch, KK Krishnadath^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Background & Aims: The metaplastic process In which the normal squamous epithelium of the distal esophagus is replaced by columnar-lined epithelium, known as Barrett's esophagus (BE), is poorly understood. The aim of this study was to define, analyze, and compare transcription profiles of BE, normal cardia epithelium, and squamous epithelium to gain more Insight into the process of metaplasia and to identify uniquely expressed genes in these epithelia. Methods: Serial analysis of gene expression was applied for obtaining transcription libraries of biopsy specimens taken from a BE-affected patient with intestinal type of metaplasia and from normal squamous and gastric cardia epithelia. Validation of results by reverse-transcription polymerase chain reaction and immunoblotting was performed using tissues of 20 patients with BE. Results: More than :120,000 tags were sequenced. Between BE and squamous 776, and between BE and gastric cardia 534 tags were significantly differentially expressed (P

Original language	English
Pages (from-to)	1274-1281
Number of pages	8
Journal	Gastroenterology
Volume	129
Issue number	4
DOIs	https://doi.org/10.1053/j.gastro.2005.07.026
Publication status	Published - Oct 2005

Keywords

FAMILY TFF PEPTIDES
INTESTINAL METAPLASIA
GASTROINTESTINAL-TRACT
CYTOKERATIN SUBSETS
CDNA MICROARRAYS
SERIAL ANALYSIS
PATTERNS
ADENOCARCINOMA
PROGRESSION
CARCINOMA

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10.1053/j.gastro.2005.07.026

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@article{e5e6ba4ef07844b0a11cdeb2782eb488,

title = "A comparative analysis by SAGE of gene expression profiles of Barrett's esophagus, normal squamous esophagus, and gastric cardia",

abstract = "Background & Aims: The metaplastic process In which the normal squamous epithelium of the distal esophagus is replaced by columnar-lined epithelium, known as Barrett's esophagus (BE), is poorly understood. The aim of this study was to define, analyze, and compare transcription profiles of BE, normal cardia epithelium, and squamous epithelium to gain more Insight into the process of metaplasia and to identify uniquely expressed genes in these epithelia. Methods: Serial analysis of gene expression was applied for obtaining transcription libraries of biopsy specimens taken from a BE-affected patient with intestinal type of metaplasia and from normal squamous and gastric cardia epithelia. Validation of results by reverse-transcription polymerase chain reaction and immunoblotting was performed using tissues of 20 patients with BE. Results: More than :120,000 tags were sequenced. Between BE and squamous 776, and between BE and gastric cardia 534 tags were significantly differentially expressed (P",

keywords = "FAMILY TFF PEPTIDES, INTESTINAL METAPLASIA, GASTROINTESTINAL-TRACT, CYTOKERATIN SUBSETS, CDNA MICROARRAYS, SERIAL ANALYSIS, PATTERNS, ADENOCARCINOMA, PROGRESSION, CARCINOMA",

author = "{Van Baal}, JWPM and F Milano and AM Rygiel and JJGHM Bergman and WD Rosmolen and {Van Deventer}, SJH and KK Wang and MP Peppelenbosch and KK Krishnadath",

year = "2005",

month = oct,

doi = "10.1053/j.gastro.2005.07.026",

language = "English",

volume = "129",

pages = "1274--1281",

journal = "Gastroenterology",

issn = "0016-5085",

publisher = "W.B. Saunders Ltd",

number = "4",

}

TY - JOUR

T1 - A comparative analysis by SAGE of gene expression profiles of Barrett's esophagus, normal squamous esophagus, and gastric cardia

AU - Van Baal, JWPM

AU - Milano, F

AU - Rygiel, AM

AU - Bergman, JJGHM

AU - Rosmolen, WD

AU - Van Deventer, SJH

AU - Wang, KK

AU - Peppelenbosch, MP

AU - Krishnadath, KK

PY - 2005/10

Y1 - 2005/10

N2 - Background & Aims: The metaplastic process In which the normal squamous epithelium of the distal esophagus is replaced by columnar-lined epithelium, known as Barrett's esophagus (BE), is poorly understood. The aim of this study was to define, analyze, and compare transcription profiles of BE, normal cardia epithelium, and squamous epithelium to gain more Insight into the process of metaplasia and to identify uniquely expressed genes in these epithelia. Methods: Serial analysis of gene expression was applied for obtaining transcription libraries of biopsy specimens taken from a BE-affected patient with intestinal type of metaplasia and from normal squamous and gastric cardia epithelia. Validation of results by reverse-transcription polymerase chain reaction and immunoblotting was performed using tissues of 20 patients with BE. Results: More than :120,000 tags were sequenced. Between BE and squamous 776, and between BE and gastric cardia 534 tags were significantly differentially expressed (P

AB - Background & Aims: The metaplastic process In which the normal squamous epithelium of the distal esophagus is replaced by columnar-lined epithelium, known as Barrett's esophagus (BE), is poorly understood. The aim of this study was to define, analyze, and compare transcription profiles of BE, normal cardia epithelium, and squamous epithelium to gain more Insight into the process of metaplasia and to identify uniquely expressed genes in these epithelia. Methods: Serial analysis of gene expression was applied for obtaining transcription libraries of biopsy specimens taken from a BE-affected patient with intestinal type of metaplasia and from normal squamous and gastric cardia epithelia. Validation of results by reverse-transcription polymerase chain reaction and immunoblotting was performed using tissues of 20 patients with BE. Results: More than :120,000 tags were sequenced. Between BE and squamous 776, and between BE and gastric cardia 534 tags were significantly differentially expressed (P

KW - FAMILY TFF PEPTIDES

KW - INTESTINAL METAPLASIA

KW - GASTROINTESTINAL-TRACT

KW - CYTOKERATIN SUBSETS

KW - CDNA MICROARRAYS

KW - SERIAL ANALYSIS

KW - PATTERNS

KW - ADENOCARCINOMA

KW - PROGRESSION

KW - CARCINOMA

U2 - 10.1053/j.gastro.2005.07.026

DO - 10.1053/j.gastro.2005.07.026

M3 - Article

C2 - 16230080

SN - 0016-5085

VL - 129

SP - 1274

EP - 1281

JO - Gastroenterology

JF - Gastroenterology

IS - 4

ER -

A comparative analysis by SAGE of gene expression profiles of Barrett's esophagus, normal squamous esophagus, and gastric cardia

Abstract

Keywords

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