A 'catch-and-release' receptor for the cholera toxin

Clare S. Mahon; Gemma C. Wildsmith; Diksha Haksar; Eyleen De Poel; Jeffrey M. Beekman; Roland J. Pieters; Michael E. Webb; W. Bruce Turnbull

doi:10.1039/c9fd00017h

A 'catch-and-release' receptor for the cholera toxin

Clare S. Mahon^*, Gemma C. Wildsmith, Diksha Haksar, Eyleen De Poel, Jeffrey M. Beekman, Roland J. Pieters, Michael E. Webb, W. Bruce Turnbull

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

1 Citation (Scopus)

Abstract

Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.

Original language	English
Pages (from-to)	112-127
Number of pages	16
Journal	Faraday discussions
Volume	219
DOIs	https://doi.org/10.1039/c9fd00017h
Publication status	Published - 30 Oct 2019

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title = "A 'catch-and-release' receptor for the cholera toxin",

abstract = "Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.",

author = "Mahon, {Clare S.} and Wildsmith, {Gemma C.} and Diksha Haksar and {De Poel}, Eyleen and Beekman, {Jeffrey M.} and Pieters, {Roland J.} and Webb, {Michael E.} and Turnbull, {W. Bruce}",

note = "Funding Information: C. S. M. wishes to thank EPSRC and the University of Leeds for the award of a Doctoral Prize Fellowship which enabled this work. W. B. T. acknowledges the support of BBSRC (BB/M005666/1). The authors thank Dr David A. Fulton, Newcastle University, UK, for access to gel permeation chromatography equipment. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2019",

month = oct,

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doi = "10.1039/c9fd00017h",

language = "English",

volume = "219",

pages = "112--127",

journal = "Faraday discussions",

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T1 - A 'catch-and-release' receptor for the cholera toxin

AU - Mahon, Clare S.

AU - Wildsmith, Gemma C.

AU - Haksar, Diksha

AU - De Poel, Eyleen

AU - Beekman, Jeffrey M.

AU - Pieters, Roland J.

AU - Webb, Michael E.

AU - Turnbull, W. Bruce

N1 - Funding Information: C. S. M. wishes to thank EPSRC and the University of Leeds for the award of a Doctoral Prize Fellowship which enabled this work. W. B. T. acknowledges the support of BBSRC (BB/M005666/1). The authors thank Dr David A. Fulton, Newcastle University, UK, for access to gel permeation chromatography equipment. Publisher Copyright: © The Royal Society of Chemistry. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2019/10/30

Y1 - 2019/10/30

N2 - Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.

AB - Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.

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U2 - 10.1039/c9fd00017h

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SN - 1359-6640

VL - 219

SP - 112

EP - 127

JO - Faraday discussions

JF - Faraday discussions

ER -

A 'catch-and-release' receptor for the cholera toxin

Abstract

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