A bioassay using intestinal organoids to measure CFTR modulators in human plasma

R Dekkers; L A W Vijftigschild; A M Vonk; E Kruisselbrink; K M  de Winter-de Groot; H M Janssens; C K van der Ent; J M Beekman; CK van der Ent

doi:10.1016/j.jcf.2014.10.007

A bioassay using intestinal organoids to measure CFTR modulators in human plasma

R Dekkers, L A W Vijftigschild, A M Vonk, E Kruisselbrink, K M de Winter-de Groot, H M Janssens, C K van der Ent, J M Beekman, CK van der Ent

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Treatment efficacies of drugs depend on patient-specific pharmacokinetic and pharmacodynamic properties. Here, we developed an assay to measure functional levels of the CFTR potentiator VX-770 in human plasma and observed that VX-770 in plasma from different donors induced variable CFTR function in intestinal organoids. This assay can help to understand variability in treatment response to CFTR potentiators by functionally modeling individual pharmacokinetics.

Original language	English
Pages (from-to)	178-181
Number of pages	4
Journal	Journal of Cystic Fibrosis
Volume	14
Issue number	2
DOIs	https://doi.org/10.1016/j.jcf.2014.10.007
Publication status	Published - 2015

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10.1016/j.jcf.2014.10.007

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title = "A bioassay using intestinal organoids to measure CFTR modulators in human plasma",

abstract = "Treatment efficacies of drugs depend on patient-specific pharmacokinetic and pharmacodynamic properties. Here, we developed an assay to measure functional levels of the CFTR potentiator VX-770 in human plasma and observed that VX-770 in plasma from different donors induced variable CFTR function in intestinal organoids. This assay can help to understand variability in treatment response to CFTR potentiators by functionally modeling individual pharmacokinetics.",

author = "R Dekkers and Vijftigschild, {L A W} and Vonk, {A M} and E Kruisselbrink and {de Winter-de Groot}, {K M} and Janssens, {H M} and {van der Ent}, {C K} and Beekman, {J M} and {van der Ent}, CK",

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AU - Dekkers, R

AU - Vijftigschild, L A W

AU - Vonk, A M

AU - Kruisselbrink, E

AU - de Winter-de Groot, K M

AU - Janssens, H M

AU - van der Ent, C K

AU - Beekman, J M

AU - van der Ent, CK

PY - 2015

Y1 - 2015

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AB - Treatment efficacies of drugs depend on patient-specific pharmacokinetic and pharmacodynamic properties. Here, we developed an assay to measure functional levels of the CFTR potentiator VX-770 in human plasma and observed that VX-770 in plasma from different donors induced variable CFTR function in intestinal organoids. This assay can help to understand variability in treatment response to CFTR potentiators by functionally modeling individual pharmacokinetics.

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DO - 10.1016/j.jcf.2014.10.007

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JO - Journal of Cystic Fibrosis

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ER -

A bioassay using intestinal organoids to measure CFTR modulators in human plasma

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