4C technology: protocols and data analysis.

Translated title of the contribution: 4C technology: protocols and data analysis.

H.J. van de Werken, P.J.P. de Vree, E. Splinter, S.J.B. Holwerda, P. Klous, E. de Wit, W. de Laat

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Chromosome conformation capture (3C) technology and its genome-wide derivatives have revolutionized our knowledge on chromatin folding and nuclear organization. 4C-seq Technology combines 3C principles with high-throughput sequencing (4C-seq) to enable for unbiased genome-wide screens for DNA contacts made by single genomic sites of interest. Here, we discuss in detail the design, application, and data analysis of 4C-seq experiments. Based on many hundreds of different 4C-seq experiments, we define criteria to assess data quality and show how different restriction enzymes and cross-linking conditions affect results. We describe in detail the mapping strategy of 4C-seq reads and show advanced strategies for data analysis.
Translated title of the contribution4C technology: protocols and data analysis.
Original languageUndefined/Unknown
Pages (from-to)89-112
Number of pages24
JournalMethods in Enzymology
Volume513
Publication statusPublished - 2012

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